New Mexico State U prof holds collaborative study with Marine Research Station

  The University’s Marine Research Station in Maribago, Mactan hosted Dr. Michele Nishiguchi, from New Mexico State University in Las Cruces, NM, and her doctoral advisee Ms. Neda Nourabadi, who collected bobtail squid species, Euprymna hyllebergi within the waters of Cebu and Bohol and sampled Vibrio fischeri, a bioluminescent bacterial symbiont found in the mantle of the said squid, from May 20 to June 1.

    The collaborative study addressed whether this symbiotic bacterium V. fischeri differ along an environmental gradient spatially by sampling populations of  both E. hyllebergi, and V. fischeri, from multiple locations in the Philippine. Initially, both Euprymna  hyllebergi and their light organ symbionts (Vibrio sp.) were collected over a 1,000 km distance, with samples taken throughout the islands of Panay, Cebu, Bohol,  Palawan, and Negros. These islands are of particular interest since the joint team discovered recently that at least three species of light organ bacteria exist in E. hyllebergi populations from Panay Island.

   At the same time, water samples were collected to isolate and test for abundance of free-living, non-symbiotic Vibrio, as well as determine the water temperature, salinity and chlorophyll content. This study also hoped to determine whether the bobtail squid, E. hyllebergi, the host populations spatial variations is reflected by symbiont diversity.

   Prof. Nishiguchi previously collected samples from Panay Island in 2010 and 2012 so that the samples collected this time in Cebu, Bohol, Negros and Palawan could be analyzed together with those from Panay.

Source:  Prof.  Michele Nishiguchi through Dr. Filipina Sotto
              USC Marine Research Station
              13 June 2013
 
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